Purification of cells and their parts
Purification of cells and their parts is achieved in the following ways:
Movement of proteins
Proteins are synthesized by ribosomes , either in the cytoplasm or in the endoplasmicreticulum (ER). Membrane proteins are synthesized in the ER andare further processed in the Golgi apparatus . From the Golgi,membrane proteins can move to the plasma membrane or to othersubcellular comparments. The ER and Golgi can be thought of as the "membrane protein synthesis compartment" and the "membraneprotein processing compartment", respectively. There is a constant flux of proteins through these compartments. ER andGolgi-resident proteins associate with other proteins and remain in their respective compartments. Other proteins "flow" throughthe ER and Golgi to the plasma membrane. From the plasma membrane, proteins destined to be degraded move back into intracellularcompartments where they are broken down to amino acids .
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